This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0024767
PrimePCR™ PreAmp for SYBR® Green Assay: LRP1, Human
PrimePCR™ Template for SYBR® Green Assay: LRP1, Human
The protein encoded by this gene is an endocytic receptor involved in several cellular processes including intracellular signaling lipid homeostasis and clearance of apoptotic cells. In addition the encoded protein is necessary for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer patients. [provided by RefSeq Jan 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.