PrimePCR™ SYBR® Green Assay: LRP1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024767

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003623
Assay Design:   Exonic
Chromosome Location:   12:57539120-57548000question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000553277 ENST00000243077 ENST00000338962 ENST00000554174

Gene Information

The protein encoded by this gene is an endocytic receptor involved in several cellular processes including intracellular signaling lipid homeostasis and clearance of apoptotic cells. In addition the encoded protein is necessary for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer patients. [provided by RefSeq Jan 2010]

Gene Symbol:   LRP1
Gene Name:   low density lipoprotein receptor-related protein 1
Aliases:   A2MR, APOER, APR, CD91, FLJ16451, IGFBP3R, LRP, MGC88725, TGFBR5
RefSeq:   NC_000012.11 NG_016444.1 NT_029419.12
Ensembl:   ENSG00000123384
Entrez:   4035
UniGene:   Hs.162757
Chromosome Mapping:   12q13-q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.360000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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