PrimePCR™ SYBR® Green Assay: BCL11B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025861

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BCL11B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BCL11B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003587
Assay Design:   Exonic
Chromosome Location:   14:99638478-99638632question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000357195 ENST00000345514

Gene Information

This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A a gene whose translocation may be associated with B-cell malignancies. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants which encode distinct isoforms have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   BCL11B
Gene Name:   B-cell CLL/lymphoma 11B (zinc finger protein)
Aliases:   CTIP-2, CTIP2, RIT1, ZNF856B, hRIT1-alpha
RefSeq:   NC_000014.8 NT_026437.12 NG_027894.1
Ensembl:   ENSG00000127152
Entrez:   64919
Chromosome Mapping:   14q32.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.300000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download