This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0024751
PrimePCR™ PreAmp for SYBR® Green Assay: PDX1, Human
PrimePCR™ Template for SYBR® Green Assay: PDX1, Human
The protein encoded by this gene is a transcriptional activator of several genes including insulin somatostatin glucokinase islet amyloid polypeptide and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM) as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.