PrimePCR™ SYBR® Green Assay: LMX1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024614

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LMX1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LMX1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003171
Assay Design:   Exonic
Chromosome Location:   9:129453220-129453309question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000526117 ENST00000373474 ENST00000355497 ENST00000425646

Gene Information

This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains 1 homeodomain and a C-terminal glutamine-rich domain. It functions as a transcription factor and is essential for the normal development of dorsal limb structures the glomerular basement membrane the anterior segment of the eye and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2010]

Gene Symbol:   LMX1B
Gene Name:   LIM homeobox transcription factor 1, beta
Aliases:   LMX1.2, MGC138325, MGC142051, NPS1
RefSeq:   NC_000009.11 NT_008470.19 NG_017039.1
Ensembl:   ENSG00000136944
Entrez:   4010
Chromosome Mapping:   9q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.780000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download