PrimePCR™ SYBR® Green Assay: CDK2AP1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003166
Assay Design:   Exonic
Chromosome Location:   12:123746132-123746309question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000261692 ENST00000538446 ENST00000535979 ENST00000542174 ENST00000544658

Gene Information

The protein encoded by this gene is a specific CDK2-associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2 and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit which suggested the regulatory role in DNA replication during S phase of the cell cycle. A similar gene in hamster was isolated from and functions as a growth suppressor of normal keratinocytes. [provided by RefSeq Jul 2008]

Gene Symbol:   CDK2AP1
Gene Name:   cyclin-dependent kinase 2 associated protein 1
Aliases:   DOC1, DORC1, ST19, doc-1, p12DOC-1
RefSeq:   NC_000012.11 NT_009755.19
Ensembl:   ENSG00000111328
Entrez:   8099
UniGene:   Hs.433201
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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