PrimePCR™ SYBR® Green Assay: CCR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024565

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CCR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CCR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002988
Assay Design:   Exonic
Chromosome Location:   3:46245541-46245662question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000296140

Gene Information

This gene encodes a member of the beta chemokine receptor family which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha) regulated on activation normal T expressed and secreted protein (RANTES) monocyte chemoattractant protein 3 (MCP-3) and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response and susceptibility to virus and parasite. This gene and other chemokine receptor genes including CCR2 CCRL2 CCR3 CCR5 and CCXCR1 are found to form a gene cluster on chromosome 3p. [provided by RefSeq Jul 2008]

Gene Symbol:   CCR1
Gene Name:   chemokine (C-C motif) receptor 1
Aliases:   CD191, CKR-1, CKR1, CMKBR1, HM145, MIP1aR, SCYAR1
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000163823
Entrez:   1230
Chromosome Mapping:   3p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.210000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download