PrimePCR™ SYBR® Green Assay: ANKS1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ANKS1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ANKS1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002916
Assay Design:   Exonic
Chromosome Location:   12:99478709-99548093question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000549558 ENST00000547776 ENST00000547010 ENST00000550693 ENST00000549025 ENST00000549493 ENST00000547446 ENST00000550778 ENST00000546568 ENST00000546960 ENST00000552245 ENST00000329257 ENST00000538702 ENST00000332712 ENST00000407362

Gene Information

This gene encodes a multi-domain protein that is predominantly expressed in brain and testis. This protein interacts with amyloid beta protein precursor (AbetaPP) and may have a role in normal brain development and in the pathogenesis of Alzheimer's disease. Expression of this gene has been shown to be elevated in patients with pre-B cell acute lymphocytic leukemia associated with t(1;19) translocation. Alternatively spliced transcript variants encoding different isoforms (some with different subcellular localization PMID:15004329) have been described for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   ANKS1B
Gene Name:   ankyrin repeat and sterile alpha motif domain containing 1B
Aliases:   AIDA, AIDA-1, ANKS2, EB-1, EB1, MGC26087, cajalin-2
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000185046
Entrez:   56899
Chromosome Mapping:   12q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.810000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download