This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: HSD11B2, Human
PrimePCR™ Template for SYBR® Green Assay: HSD11B2, Human
There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme encoded by this gene has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor such as the placenta and testis it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq Feb 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.