PrimePCR™ SYBR® Green Assay: BCAR1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002784
Assay Design:   Exonic
Chromosome Location:   16:75268881-75269010question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000162330 ENST00000393422 ENST00000420641 ENST00000538440 ENST00000418647 ENST00000535626 ENST00000393420 ENST00000542031 ENST00000546196

Gene Information

BCAR1 or CAS is an Src (MIM 190090) family kinase substrate involved in various cellular events including migration survival transformation and invasion (Sawada et al. 2006 [PubMed 17129785]).[supplied by OMIM May 2009]

Gene Symbol:   BCAR1
Gene Name:   breast cancer anti-estrogen resistance 1
Aliases:   CAS, CAS1, CASS1, CRKAS, FLJ12176, FLJ45059, P130Cas
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000050820
Entrez:   9564
UniGene:   Hs.479747
Chromosome Mapping:   16q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.920000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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