PrimePCR™ SYBR® Green Assay: RPH3A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002728
Assay Design:   Exonic
Chromosome Location:   12:113285535-113285624question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000548197 ENST00000547686 ENST00000543106 ENST00000551593 ENST00000551748 ENST00000546703 ENST00000547840 ENST00000547728 ENST00000549769 ENST00000552667 ENST00000389385 ENST00000550901 ENST00000551198 ENST00000551052 ENST00000415485 ENST00000553114 ENST00000420983

Gene Information

Exocytosis of neurotransmitters and hormones is fundamental to synaptic neurotransmission and cell-cell communication. RAB3A (MIM 179390) is a small G protein that is thought to act at late stages of exocytosis and RPH3A is a RAB3A effector (Lin et al. 2007 [PubMed 17149709]).[supplied by OMIM Jul 2008]

Gene Symbol:   RPH3A
Gene Name:   rabphilin 3A homolog (mouse)
Aliases:   KIAA0985
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000089169
Entrez:   22895
UniGene:   Hs.21239
Chromosome Mapping:   12q24.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.380000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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