PrimePCR™ SYBR® Green Assay: RAX, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024485

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002715
Assay Design:   Exonic
Chromosome Location:   18:56935186-56935305question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000256852 ENST00000334889

Gene Information

This gene encodes a homeobox-containing transcription factor that functions in eye development. The gene is expressed early in the eye primordia and is required for retinal cell fate determination and also regulates stem cell proliferation. Mutations in this gene have been reported in patients with defects in ocular development including microphthalmia anophthalmia and coloboma.[provided by RefSeq Oct 2009]

Gene Symbol:   RAX
Gene Name:   retina and anterior neural fold homeobox
Aliases:   MCOP3, RX
RefSeq:   NC_000018.9 NG_013031.1 NT_025028.14
Ensembl:   ENSG00000134438
Entrez:   30062
UniGene:   Hs.278957
Chromosome Mapping:   18q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.630000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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