This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0024409
PrimePCR™ PreAmp for SYBR® Green Assay: PRM2, Human
PrimePCR™ Template for SYBR® Green Assay: PRM2, Human
Protamines are the major DNA-binding proteins in the nucleus of sperm and package the DNA in a volume less than 5% of a somatic cell nucleus (summarized by Cho et al. 2001 [PubMed 11326282]). See PRM1 (MIM 182880).[supplied by OMIM Jul 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.