This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0024386
PrimePCR™ PreAmp for SYBR® Green Assay: CHTF18, Human
PrimePCR™ Template for SYBR® Green Assay: CHTF18, Human
CHTF18 CHTF8 (MIM 613202) and DCC1 (DSCC1; MIM 613203) are components of an alternative replication factor C (RFC) (see MIM 600404) complex that loads PCNA (MIM 176740) onto DNA during S phase of the cell cycle (Merkle et al. 2003 [PubMed 12766176]; Bermudez et al. 2003 [PubMed 12930902]).[supplied by OMIM Dec 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.