This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: DCAF12L2, Human
PrimePCR™ Template for SYBR® Green Assay: DCAF12L2, Human
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by Gly-His and Trp-Asp (GH-WD) which may facilitate formation of heterotrimeric or multi-protein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression signal transduction apoptosis and gene regulation. This gene appears to represent an intronless retrocopy of a related multi-exon gene located on chromosome 9. However the CDS of this intronless gene remains intact it is conserved in other mammalian species it is known to be transcribed and it is therefore thought to encode a functional protein. [provided by RefSeq May 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.