PrimePCR™ SYBR® Green Assay: TAS1R3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024347

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002321
Assay Design:   Exonic
Chromosome Location:   1:1267196-1267414question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000339381

Gene Information

The TAS1R3 gene encodes the human homolog of mouse Sac a major determinant of differences between sweet-sensitive and -insensitive mouse strains in their responsiveness to sucrose saccharine and other sweeteners (Max et al. 2001 [PubMed 11326277]).[supplied by OMIM Jan 2010]

Gene Symbol:   TAS1R3
Gene Name:   taste receptor, type 1, member 3
Aliases:   T1R3
RefSeq:   NC_000001.10 NG_008048.1 NT_004350.19
Ensembl:   ENSG00000169962
Entrez:   83756
UniGene:   Hs.74375
Chromosome Mapping:   1p36.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.800000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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