PrimePCR™ SYBR® Green Assay: SGK1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002300
Assay Design:   Exonic
Chromosome Location:   6:134495649-134528510question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000367858 ENST00000413996 ENST00000237305 ENST00000367857 ENST00000528577 ENST00000475719 ENST00000461976

Gene Information

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium sodium and chloride channels suggesting an involvement in the regulation of processes such as cell survival neuronal excitability and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Jan 2009]

Gene Symbol:   SGK1
Gene Name:   serum/glucocorticoid regulated kinase 1
Aliases:   SGK
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000118515
Entrez:   6446
UniGene:   Hs.510078
Chromosome Mapping:   6q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.810000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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