This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0024300
PrimePCR™ PreAmp for SYBR® Green Assay: HR, Human
PrimePCR™ Template for SYBR® Green Assay: HR, Human
This gene encodes a protein that is involved in hair growth. This protein functions as a transcriptional corepressor of multiple nuclear receptors including thyroid hormone receptor the retinoic acid receptor-related orphan receptors and the vitamin D receptors and it interacts with histone deacetylases. The translation of this protein is modulated by multiple regulatory ORFs that exist upstream of the primary ORF. Mutations in one of these upstream ORFs U2HR cause Marie Unna hereditary hypotrichosis (MUHH) an autosomal dominant form of genetic hair loss. Mutations in this gene also cause autosomal recessive congenital alopecia and atrichia with papular lesions other diseases resulting in hair loss. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.