PrimePCR™ SYBR® Green Assay: BBX, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BBX, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BBX, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002173
Assay Design:   Exonic
Chromosome Location:   3:107429372-107429465question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000415149 ENST00000402543 ENST00000325805 ENST00000427402 ENST00000416476 ENST00000431630 ENST00000449335 ENST00000456817 ENST00000458458 ENST00000437908 ENST00000456419 ENST00000402163 ENST00000406780 ENST00000413213 ENST00000449271 ENST00000449213 ENST00000457496 ENST00000429270 ENST00000325767

Gene Information

Description Not Available

Gene Symbol:   BBX
Gene Name:   bobby sox homolog (Drosophila)
Aliases:   HBP2, HSPC339, MDS001
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114439
Entrez:   56987
Chromosome Mapping:   3q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.370000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download