PrimePCR™ SYBR® Green Assay: BTK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002139
Assay Design:   Exonic
Chromosome Location:   X:100608194-100608296question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000372880 ENST00000308731 ENST00000372855 ENST00000372859 ENST00000372860 ENST00000372869 ENST00000443591 ENST00000540426

Gene Information

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1 which is an immunodeficiency characterized by the failure to produce mature B lymphocytes and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq Nov 2008]

Gene Symbol:   BTK
Gene Name:   Bruton agammaglobulinemia tyrosine kinase
Aliases:   AGMX1, AT, ATK, BPK, IMD1, MGC126261, MGC126262, PSCTK1, XLA
RefSeq:   NC_000023.10 NG_009616.1 NG_011734.1 NG_012523.1 NT_011651.17
Ensembl:   ENSG00000010671
Entrez:   695
UniGene:   Hs.159494
Chromosome Mapping:   Xq21.33-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 35.010000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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