This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0024274
PrimePCR™ PreAmp for SYBR® Green Assay: NKX6-3, Human
PrimePCR™ Template for SYBR® Green Assay: NKX6-3, Human
The NKX family of homeodomain proteins controls numerous developmental processes. Members of the NKX6 subfamily including NKX6-3 are involved in development of the central nervous system (CNS) gastrointestinal tract and pancreas (Alanentalo et al. 2006 [PubMed 16326147]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.