This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0024271
PrimePCR™ PreAmp for SYBR® Green Assay: TRAF4, Human
PrimePCR™ Template for SYBR® Green Assay: TRAF4, Human
This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor p75 (NTR/NTSR1) and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.