This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: LAGE3, Human
PrimePCR™ Template for SYBR® Green Assay: LAGE3, Human
This gene belongs to the ESO/LAGE gene family members of which are clustered together on chromosome Xq28 and have similar exon-intron structures. Unlike the other family members which are normally expressed only in testis and activated in a wide range of human tumors this gene is ubiquitously expressed in somatic tissues. The latter combined with the finding that it is highly conserved in mouse and rat suggests that the encoded protein is functionally important. An intronless pseudogene with high sequence similarity to this gene is located on chromosome 9. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.