PrimePCR™ SYBR® Green Assay: DAK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024262

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DAK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DAK, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002059
Assay Design:   Exonic
Chromosome Location:   11:61113823-61113980question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000394900 ENST00000529479

Gene Information

This gene is a member of the family of dihydroxyacetone kinases which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone and also catalyzes the formation of riboflavin 4'5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified but the full-length nature of only one has been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   DAK
Gene Name:   dihydroxyacetone kinase 2 homolog (S. cerevisiae)
Aliases:   DKFZp586B1621, MGC5621, NET45
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000149476
Entrez:   26007
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.420000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download