This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0024242
PrimePCR™ PreAmp for SYBR® Green Assay: RBM15B, Human
PrimePCR™ Template for SYBR® Green Assay: RBM15B, Human
Members of the SPEN (Split-end) family of proteins including RBM15B have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al. 2005 [PubMed 16129689]).[supplied by OMIM Feb 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.