PrimePCR™ SYBR® Green Assay: ARAP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025813

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARAP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARAP3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001982
Assay Design:   Exonic
Chromosome Location:   5:141053221-141053346question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000522690 ENST00000508305 ENST00000239440 ENST00000504448

Gene Information

This gene encodes a phosphoinositide binding protein containing ARF-GAP RHO-GAP RAS-associating and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(345)P3/PtdIns(34)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene but its biological validity has not been determined. [provided by RefSeq Sep 2008]

Gene Symbol:   ARAP3
Gene Name:   ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
Aliases:   CENTD3, DRAG1, FLJ21065
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000120318
Entrez:   64411
Chromosome Mapping:   5q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.310000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download