This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: TRIM7, Human
PrimePCR™ Template for SYBR® Green Assay: TRIM7, Human
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains a RING a B-box type 1 a B-box type 2 and a coiled-coil region. The protein localizes to both the nucleus and the cytoplasm and may represent a participant in the initiation of glycogen synthesis. Multiple transcript variants have been found for this gene and some of them encode the same isoform. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.