PrimePCR™ SYBR® Green Assay: EPB41L2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EPB41L2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EPB41L2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001777
Assay Design:   Exonic
Chromosome Location:   6:131277501-131277603question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000528282 ENST00000530481 ENST00000445890 ENST00000337057 ENST00000392427 ENST00000368128 ENST00000527411 ENST00000525271 ENST00000525193 ENST00000527659 ENST00000529208 ENST00000529709 ENST00000532499 ENST00000526983 ENST00000531356 ENST00000530707 ENST00000257986 ENST00000425439

Gene Information

Description Not Available

Gene Symbol:   EPB41L2
Gene Name:   erythrocyte membrane protein band 4.1-like 2
Aliases:   4.1-G, DKFZp781D1972, DKFZp781H1755
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000079819
Entrez:   2037
Chromosome Mapping:   6q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.270000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download