PrimePCR™ SYBR® Green Assay: cast, Zebrafish

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: cast, Zebrafish
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: cast, Zebrafish
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qDreCID0025771
Assay Design:   Intron-spanning
Chromosome Location:   21:10031784-10033264question
Amplicon Length:   69
Splice Variants Targeted:   ENSDART00000132699 ENSDART00000139814 ENSDART00000135937 ENSDART00000148097 ENSDART00000138619 ENSDART00000141297 ENSDART00000144770 ENSDART00000136308 ENSDART00000133443 ENSDART00000081614

Gene Information

Description Not Available

Gene Symbol:   cast
Gene Name:   Calpastatin
Aliases:   Not Available
RefSeq:   XM_005161036 XM_005161035 XM_005161026 XM_005161032 XM_005161025 XM_005161044 XM_005161041 XM_005161039 XM_005161038 XM_005161029 XM_005161043 XM_005161034 XM_005161051 XM_005161049 XM_005161031 XM_005161027 XM_005161030 XM_005161028 XM_005161050 XM_005161046 XM_005161045 XM_005161040 XM_005161037 XM_005161047 XM_005161033 XM_005161042 XM_005161048 NM_001130591
Ensembl:   ENSDARG00000058693
Entrez:   563084
Chromosome Mapping:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download