PrimePCR™ SYBR® Green Assay: OAZ3, Dog

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: OAZ3, Dog
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: OAZ3, Dog
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qCfaCID0030135
Assay Design:   Intron-spanning
Chromosome Location:   17:60791750-60792783question
Amplicon Length:   80
Splice Variants Targeted:   ENSCAFT00000020375

Gene Information

The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamine in cells. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis pathway; thus, completing the auto-regulatory circuit. This gene encodes antizyme 3, the third member of the antizyme family. Like antizymes 1 and 2, antizyme 3 inhibits ODC activity and polyamine uptake, however, it does not stimulate ODC degradation. Also, while antizymes 1 and 2 have broad tissue distribution, expression of antizyme 3 is restricted to haploid germ cells in testis, suggesting a distinct role for this antizyme in spermiogenesis. Antizyme 3 gene knockout studies in mice showed that homozygous mutant male mice were infertile, and indicated the likely role of this antizyme in the formation of a rigid connection between the sperm head and tail during spermatogenesis. This transcript initiates translation from a non-AUG (CUG) start codon that is highly conserved amongst the antizyme 3 orthologs. [provided by RefSeq, Aug 2014]

Gene Symbol:   OAZ3
Gene Name:   Canis lupus familiaris ornithine decarboxylase antizyme 3 (OAZ3), mRNA.
Aliases:   Not Available
RefSeq:   NM_001134951
Ensembl:   ENSCAFG00000012840
Entrez:   475850
Chromosome Mapping:   17

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download