PrimePCR™ SYBR® Green Assay: GTF3A, Cow

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: GTF3A, Cow
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GTF3A, Cow
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qBtaCID0012253
Assay Design:   Intron-spanning
Chromosome Location:   12:32725878-32728266question
Amplicon Length:   82
Splice Variants Targeted:   ENSBTAT00000016732

Gene Information

The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. [provided by RefSeq, Jul 2008]

Gene Symbol:   GTF3A
Gene Name:   General transcription factor IIIA
Aliases:   Not Available
RefSeq:   XM_005213759 NM_001113572
Ensembl:   ENSBTAG00000012600
Entrez:   100135054
Chromosome Mapping:   12

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download