PrimePCR™ Probe Assay: WRKY18, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WRKY18, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WRKY18, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0026685
Assay Design:   Exonic
Chromosome Location:   4:15383924-15384048question
Amplicon Length:   95
Splice Variants Targeted:   AT5G25590.1 AT4G31800.1 AT4G31800.2

Gene Information

Pathogen-induced transcription factor. Binds W-box sequences in vitro. Forms protein complexes with itself and with WRKY40 and WRKY60. Constitutive expression of WRKY18 enhanced resistance to P. syringae, but its coexpression with WRKY40 or WRKY60 made plants more susceptible to both P. syringae and B. cinerea. WRKY18, WRKY40, and WRKY60 have partially redundant roles in response to the hemibiotrophic bacterial pathogen Pseudomonas syringae and the necrotrophic fungal pathogen Botrytis cinerea, with WRKY18 playing a more important role than the other two.

Gene Symbol:   WRKY18
Gene Name:   WRKY DNA-binding protein 18
Aliases:   Not Available
RefSeq:   NM_119329 NM_001036689
Ensembl:   AT4G31800
Entrez:   829308
Chromosome Mapping:   Not Available

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