PrimePCR™ Probe Assay: APG8A, Arabidopsis

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0026445
Assay Design:   Exonic
Chromosome Location:   4:11655674-11655782question
Amplicon Length:   79
Splice Variants Targeted:   AT5G23380.1 AT4G21980.1 AT4G21980.2

Gene Information

Encodes APG8, a component of autophagy conjugation pathway. Delivered to the lumens of vacuole under nitrogen-starvation condition. Highest expression in flowers. mRNA abundance increased during dark-induced carbon starvation. Predominantly cytoplasmic with or without N starvation. Upon concanamycin A the protein accumulates in the central vacuole as punctuate structures that resemble autophagic bodies. This localization is more abundant upon N starvation.

Gene Symbol:   APG8A
Gene Name:   Ubiquitin-like superfamily protein
Aliases:   Not Available
RefSeq:   NM_118319 NM_001084955
Ensembl:   AT4G21980
Entrez:   828287
UniGene:   At.2106,At.24034
Chromosome Mapping:   Not Available

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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