PrimePCR™ Probe Assay: SSP4b, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SSP4b, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SSP4b, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0026363
Assay Design:   Exonic
Chromosome Location:   4:10048211-10048315question
Amplicon Length:   75
Splice Variants Targeted:   AT4G18140.1 AT4G18140.2 AT4G18140.3 AT1G78300.1

Gene Information

Encodes a SCP1-like small phosphatase (SSP). Three SSPs form a unique group with long N-terminal extensions: AT5G46410 (SSP4), AT5G11860 (SSP5), AT4G18140 (SSP4b). SSP4 and SSP4b were localized exclusively in the nuclei, whereas SSP5 accumulated in both nuclei and cytoplasm. All three SSPs encodes active CTD phosphatases like animal SCP1 family proteins, with distinct substrate specificities: SSP4 and SSP4b could dephosphorylate both Ser2-PO(4) and Ser5-PO(4) of CTD, whereas SSP5 dephosphorylated only Ser5-PO(4).

Gene Symbol:   SSP4b
Gene Name:   SCP1-like small phosphatase 4b
Aliases:   Not Available
RefSeq:   NM_001036584 NM_117924 NM_001203831
Ensembl:   AT4G18140
Entrez:   827539
Chromosome Mapping:   Not Available

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