PrimePCR™ Probe Assay: PPD1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PPD1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPD1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0026298
Assay Design:   Exonic
Chromosome Location:   4:8426781-8427000question
Amplicon Length:   190
Splice Variants Targeted:   AT4G14713.1 AT4G14713.2 AT1G72430.1

Gene Information

PPD1 (and its paralog, PPD2) encode plant-specific putative DNA-binding proteins. PPD1 and PPD2 are not found in grasses. Overexpression of PPD reduces lamina size by promoting the early arrest of dispersed meristematic cells DMC proliferation during leaf and silique development. Deletion of the PPD locus increases leaf lamina size and results in dome-shaped rather than flat leaves. Siliques are also altered in shape because of extra lamina growth. The curvature of a deltappd leaf reflects the difference between excess growth of the lamina and a limitation to the extension capacity of its perimeter.

Gene Symbol:   PPD1
Gene Name:   TIFY domain/Divergent CCT motif family protein
Aliases:   Not Available
RefSeq:   NM_117555 NM_202819
Ensembl:   AT4G14713
Entrez:   827123
Chromosome Mapping:   Not Available

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