PrimePCR™ Probe Assay: UBQ10, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: UBQ10, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: UBQ10, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0026151
Assay Design:   Exonic
Chromosome Location:   4:2719604-2719743question
Amplicon Length:   110
Splice Variants Targeted:   AT4G05050.1 AT4G05050.2 AT4G05050.3 AT4G05320.1 AT4G05320.2 AT4G05320.3 AT4G05320.4 AT4G05320.5 AT4G05320.6 AT3G09790.1

Gene Information

One of five polyubiquitin genes in A. thaliana. These genes encode the highly conserved 76-amino acid protein ubiquitin that is covalently attached to substrate proteins targeting most for degradation. Polyubiquitin genes are characterized by the presence of tandem repeats of the 228 bp that encode a ubiquitin monomer. Induced by salicylic acid. Independent of NPR1 for their induction by salicylic acid.

Gene Symbol:   UBQ10
Gene Name:   Polyubiquitin 10
Aliases:   Not Available
RefSeq:   NM_178968 NM_116771 NM_202787 NM_001084884 NM_178969 NM_178970
Ensembl:   AT4G05320
Entrez:   825880
Chromosome Mapping:   Not Available

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