PrimePCR™ Probe Assay: MSH6, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MSH6, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MSH6, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0026100
Assay Design:   Exonic
Chromosome Location:   4:906716-906864question
Amplicon Length:   119
Splice Variants Targeted:   AT4G02070.1 AT4G02070.2 AT1G02030.1

Gene Information

encodes a DNA mismatch repair homolog of human MutS gene, MSH6. There are four MutS genes in Arabidopsis, MSH2, MSH3, MSH6, and MSH7, which all act as heterodimers and bind to 51-mer duplexes. MSH2*MSH6 bound the (+T) substrate strongly, (T/G) well, and (+AAG) no better than it did a (T/A) homoduplex.

Gene Symbol:   MSH6
Gene Name:   MUTS homolog 6
Aliases:   Not Available
RefSeq:   NM_001203727 NM_116438
Ensembl:   AT4G02070
Entrez:   828147
Chromosome Mapping:   Not Available

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