PrimePCR™ Probe Assay: NRPD2A, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0025531
Assay Design:   Exonic
Chromosome Location:   3:8574020-8574147question
Amplicon Length:   98
Splice Variants Targeted:   AT5G11630.1 AT3G23780.1 AT3G23780.2

Gene Information

This gene encodes the second largest, catalytic subunit of the nuclear DNA-dependent RNA polymerase IV (aka RNA polymerase D). The NRPD2 protein is found at nuclear foci that overlap or are adjacent to chromocentromeres but are not fully coincident with chromocentromeres. The loss of NRPD2 leads to the loss of cytosine methylation at pericentromeric 5S genes and AtSN1 retroelements but has no discernible effect on centromere repeat methylation. This suggests that Pol IV primarily affects facultative heterochromatin rather than constitutive heterochromatin.

Gene Symbol:   NRPD2A
Gene Name:   Nuclear RNA polymerase D2A
Aliases:   Not Available
RefSeq:   NM_001203028 NM_113282
Ensembl:   AT3G23780
Entrez:   821960
UniGene:   At.37653
Chromosome Mapping:   Not Available

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