PrimePCR™ Probe Assay: NRPD2A, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NRPD2A, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NRPD2A, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0025531
Assay Design:   Exonic
Chromosome Location:   3:8574020-8574147question
Amplicon Length:   98
Splice Variants Targeted:   AT5G11630.1 AT3G23780.1 AT3G23780.2

Gene Information

This gene encodes the second largest, catalytic subunit of the nuclear DNA-dependent RNA polymerase IV (aka RNA polymerase D). The NRPD2 protein is found at nuclear foci that overlap or are adjacent to chromocentromeres but are not fully coincident with chromocentromeres. The loss of NRPD2 leads to the loss of cytosine methylation at pericentromeric 5S genes and AtSN1 retroelements but has no discernible effect on centromere repeat methylation. This suggests that Pol IV primarily affects facultative heterochromatin rather than constitutive heterochromatin.

Gene Symbol:   NRPD2A
Gene Name:   Nuclear RNA polymerase D2A
Aliases:   Not Available
RefSeq:   NM_001203028 NM_113282
Ensembl:   AT3G23780
Entrez:   821960
Chromosome Mapping:   Not Available

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