PrimePCR™ Probe Assay: JAZ3, Arabidopsis

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0025427
Assay Design:   Exonic
Chromosome Location:   3:6122076-6122168question
Amplicon Length:   63
Splice Variants Targeted:   AT3G17860.1 AT3G17860.2 AT3G17860.3

Gene Information

JAZs are direct targets of the SCFCOI1 E3 ubiquitin-ligase and JA treatment induces their proteasome-mediated degradation. Furthermore, JAI3 negatively regulates the key transcriptional activator of JA responses, AtMYC2. The C-terminal portion of JAZ3, including the Jas domain, appears to be important for JAZ3-COI1 binding in the presence of coronatine.

Gene Symbol:   JAZ3
Gene Name:   Jasmonate-zim-domain protein 3
Aliases:   Not Available
RefSeq:   NM_112667 NM_001084705 NM_202601
Ensembl:   AT3G17860
Entrez:   821055
UniGene:   At.8035
Chromosome Mapping:   Not Available

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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