PrimePCR™ Probe Assay: JAR1, Arabidopsis

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0025038
Assay Design:   Exonic
Chromosome Location:   2:19036058-19036207question
Amplicon Length:   120
Splice Variants Targeted:   AT5G06120.2 AT5G06120.3 AT5G06120.4 AT2G46370.1 AT2G46370.2 AT2G46370.3 AT2G46370.4

Gene Information

Encodes a jasmonate-amido synthetase that is a member of the GH3 family of proteins. JAR1 catalyzes the formation of a biologically active jasmonyl-isoleucine (JA-Ile) conjugate. JA-Ile promotes the interaction between JAZ1 and COI1 in the jasmonate signaling pathway. JAR1 localizes to the cytoplasm and is also a phytochrome A signaling component. JAR1 is an auxin-induced gene. Loss of function mutants are defective in a variety of responses to jasmonic acid. JAR1 has additional enzymatic activities in vitro, (e.g. the ability to synthesize adenosine 5'-tetraphosphate and other JA conjugates), but there are no data to show whether JAR1 catalyzes many of these reactions in vivo. JAR1 is involved in pathogen defense, sensitivity to ozone, and wound responses.

Gene Symbol:   JAR1
Gene Name:   Auxin-responsive GH3 family protein
Aliases:   Not Available
RefSeq:   NM_001202828 NM_180122 NM_130200 NM_001084600
Ensembl:   AT2G46370
Entrez:   819244
UniGene:   At.13902
Chromosome Mapping:   Not Available

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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