PrimePCR™ Probe Assay: PIL5, Arabidopsis


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0024480
Assay Design:   Exonic
Chromosome Location:   2:8706135-8706268question
Amplicon Length:   104
Splice Variants Targeted:   AT5G40450.1 AT2G20180.1 AT2G20180.2 AT2G20180.3

Gene Information

Encodes a novel Myc-related bHLH transcription factor that has transcriptional activation activity in the dark. It is a key negative regulator of phytochrome-mediated seed germination and acts by inhibiting chlorophyll biosynthesis, light-mediated suppression of hypocotyl elongation and far-red light-mediated suppression of seed germination, and promoting negative gravitropism in hypocotyls. Light reduces this activity in a phy-dependent manner. The protein preferentially interacts with the Pfr forms of Phytochrome A (PhyA) and Phytochrome B (PhyB), is physically associated with APRR1/TOC1 and is degraded in red (R) and far-red (FR) light through the ubiquitin (ub)-26S proteasome pathway to optimize photomorphogenic development in Arabidopsis. It also negatively regulates GA3 oxidase expression.

Gene Symbol:   PIL5
Gene Name:   Phytochrome interacting factor 3-like 5
Aliases:   Not Available
RefSeq:   NM_179665 NM_127577 NM_001202630
Ensembl:   AT2G20180
Entrez:   816538
UniGene:   At.43003,At.69322
Chromosome Mapping:   Not Available

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