PrimePCR™ Probe Assay: AtPOT1a, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AtPOT1a, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AtPOT1a, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0024310
Assay Design:   Exonic
Chromosome Location:   2:1894022-1894194question
Amplicon Length:   143
Splice Variants Targeted:   AT2G05210.1 AT2G05210.2 AT2G05210.3 AT1G05320.1 AT1G05320.2 AT1G05320.3

Gene Information

Encodes AtPOT1a, an accessory factor for telomerase required for positive telomere length regulation. Note on nomenclature: different names have been given to Arabidopsis POT-like genes (Kuchar and Fajkus, 2004; Shakirov et al, 2005; Tani and Murata, 2005). According to a unifying nomenclature (Surovtseva et al, 2007), At2g05210 (previously named AtPOT1) is designated AtPOT1a, while At5g06310 (previously named AtPOT2) is designated AtPOT1b.

Gene Symbol:   AtPOT1a
Gene Name:   Nucleic acid-binding, OB-fold-like protein
Aliases:   Not Available
RefSeq:   NM_001124799 NM_126547 NM_001124798
Ensembl:   AT2G05210
Entrez:   815069
Chromosome Mapping:   Not Available

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