PrimePCR™ Probe Assay: UBC35, Arabidopsis

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0024172
Assay Design:   Exonic
Chromosome Location:   1:29650500-29650747question
Amplicon Length:   120
Splice Variants Targeted:   AT4G22350.1 AT4G22350.2 AT4G38070.1 AT3G17100.1 AT3G17100.2 AT2G22680.1 AT1G78870.1 AT1G78870.2 AT1G78870.3

Gene Information

UBC35/UBC13A encodes a protein that may play a role in DNA damage responses and error-free post-replicative DNA repair by participating in lysine-63-based polyubiquitination reactions. UBC35/UBC13A can form diubiquitin and triubiquitin chains in combination with MMZ1,2,3,4/(UEV1A,B,C,D) in vitro. It can also functionally complement an mms2 ubc13 mutation in budding yeast by increasing the double mutant's viability in the presence of the DNA damaging agent MMS, when it is co-expressed with MMZ / UEV1 genes. A wild type phenotype is restored with MMZ3/UEV1C and MMZ4/UEV1D, but only partial complementation is achieved with MMZ1/UEV1A or MMZ2/UEV1B.

Gene Symbol:   UBC35
Gene Name:   Ubiquitin-conjugating enzyme 35
Aliases:   Not Available
RefSeq:   NM_106535 NM_001036221 NM_179571
Ensembl:   AT1G78870
Entrez:   844224
UniGene:   At.46456
Chromosome Mapping:   Not Available

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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