PrimePCR™ Probe Assay: AVP1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AVP1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AVP1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0023134
Assay Design:   Exonic
Chromosome Location:   1:5399233-5399347question
Amplicon Length:   85
Splice Variants Targeted:   AT5G45150.1 AT1G15690.1 AT1G15690.2

Gene Information

Encodes a H(+)-translocating (pyrophosphate-energized) inorganic pyrophosphatase (H(+)-PPase; EC 3.6.1.1) located in the vacuolar membrane. Expression is found in all tissues examined, including meristems and floral organ primordium. Expression is particularly enhanced in pollen, and is repressed by light. Over expression and loss of function phenotypes suggest AVP1 is involved in regulation of apoplastic pH and auxin transport. The effect on auxin transport likely involves effects of extracellular pH on subcellular localization of auxin efflux carriers such as PIN1.

Gene Symbol:   AVP1
Gene Name:   Inorganic H pyrophosphatase family protein
Aliases:   Not Available
RefSeq:   NM_001084073 NM_101437
Ensembl:   AT1G15690
Entrez:   838138
Chromosome Mapping:   Not Available

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