PrimePCR™ Probe Assay: CRY2, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0023005
Assay Design:   Exonic
Chromosome Location:   1:1186836-1186962question
Amplicon Length:   97
Splice Variants Targeted:   AT1G04400.1 AT1G04400.2

Gene Information

Blue light receptor mediating blue-light regulated cotyledon expansion and flowering time. Positive regulator of the flowering-time gene CONSTANS. This gene possesses a light-induced CNT2 N-terminal homodimerisation domain.Involved in blue-light induced stomatal opening. Involved in triggering chromatin decondensation. An 80-residue motif (NC80) is sufficient to confer CRY2's physiological function. It is proposed that the PHR domain and the C-terminal tail of the unphosphorylated CRY2 form a "closed" conformation to suppress the NC80 motif in the absence of light. In response to blue light, the C-terminal tail of CRY2 is phosphorylated and electrostatically repelled from the surface of the PHR domain to form an "open" conformation, resulting in derepression of the NC80 motif and signal transduction to trigger photomorphogenic responses. Cry2 phosphorylation and degradation both occur in the nucleus.

Gene Symbol:   CRY2
Gene Name:   Cryptochrome 2
Aliases:   Not Available
RefSeq:   NM_100320 NM_179257
Ensembl:   AT1G04400
Entrez:   839529
UniGene:   At.21976
Chromosome Mapping:   Not Available

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