PrimePCR™ Probe Assay: APX1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: APX1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: APX1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0022933
Assay Design:   Exonic
Chromosome Location:   1:2438096-2438330question
Amplicon Length:   118
Splice Variants Targeted:   AT4G26520.1 AT1G07890.1 AT1G07890.2 AT1G07890.3 AT1G07890.4 AT1G07890.5 AT1G07890.6 AT1G07890.7 AT1G07890.8

Gene Information

Encodes a cytosolic ascorbate peroxidase APX1. Ascorbate peroxidases are enzymes that scavenge hydrogen peroxide in plant cells. Eight types of APX have been described for Arabidopsis: three cytosolic (APX1, APX2, APX6), two chloroplastic types (stromal sAPX, thylakoid tAPX), and three microsomal (APX3, APX4, APX5) isoforms. At least part of the induction of heat shock proteins during light stress in Arabidopsis is mediated by H2O2 that is scavenged by APX1. Expression of the gene is downregulated in the presence of paraquat, an inducer of photoxidative stress.

Gene Symbol:   APX1
Gene Name:   Ascorbate peroxidase 1
Aliases:   Not Available
RefSeq:   NM_001084012 NM_100663 NM_179276 NM_001035914 NM_001084013 NM_001035915 NM_001123772 NM_202057
Ensembl:   AT1G07890
Entrez:   837304
Chromosome Mapping:   Not Available

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