PrimePCR™ Probe Assay: MLO2, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0022858
Assay Design:   Exonic
Chromosome Location:   1:3800982-3801118question
Amplicon Length:   107
Splice Variants Targeted:   AT4G34440.1 AT1G11310.1 AT1G11310.2

Gene Information

A member of a large family of seven-transmembrane domain proteins specific to plants, homologs of the barley mildew resistance locus o (MLO) protein. The Arabidopsis genome contains 15 genes encoding MLO proteins, with localization in plasma membrane. Phylogenetic analysis revealed four clades of closely-related AtMLO genes. ATMLO2 belongs to the clade IV, with AtMLO3, AtMLO6 and AtMLO12. The gene is expressed during early seedling growth, in roots, in vascular system of cotyledons and young leaves,and in fruit abscission zone; it was not expressed in anthers and pollen, as shown by GUS activity patterns. The expression of several phylogenetically closely-related AtMLO genes showed similar or overlapping tissue specificity and analogous responsiveness to external stimuli, suggesting functional redundancy, co-function, or antagonistic function(s). mlo resistance in A. thaliana does not involve the signaling molecules ethylene, jasmonic acid or salicylic acid, but requires a syntaxin, glycosyl hydrolase and ABC transporter.

Gene Symbol:   MLO2
Gene Name:   Seven transmembrane MLO family protein
Aliases:   Not Available
RefSeq:   NM_101004 NM_001123796
Ensembl:   AT1G11310
Entrez:   837673
UniGene:   At.24513
Chromosome Mapping:   Not Available

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