PrimePCR™ Probe Assay: ACT12, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ACT12, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ACT12, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0020854
Assay Design:   Exonic
Chromosome Location:   3:17128709-17128855question
Amplicon Length:   117
Splice Variants Targeted:   AT3G46520.1

Gene Information

Member of actin subclass composed of ACT12 and ACT4. RNA is expressed at very low levels in vegetative organs, low levels in flowers and very high levels in pollen. Expression of an ACT12/GUS fusion was found in vascular tissues, tapetum, developing and mature pollen, the root cap and in a ring of pericycle tissues during lateral root initiation and early development.

Gene Symbol:   ACT12
Gene Name:   Actin-12
Aliases:   Not Available
RefSeq:   NM_114519
Ensembl:   AT3G46520
Entrez:   823805
Chromosome Mapping:   Not Available

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