PrimePCR™ Probe Assay: ATH1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATH1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATH1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0018163
Assay Design:   Exonic
Chromosome Location:   4:15916525-15916651question
Amplicon Length:   97
Splice Variants Targeted:   AT4G32980.1

Gene Information

Encodes transcription factor involved in photomorphogenesis. Regulates gibberellin biosynthesis. Activated by AGAMOUS in a cal-1, ap1-1 background. Expressed at low levels in developing stamens. Increased levels of ATH1 severely delay flowering in the C24 accession. Most remarkably, ectopically expressed ATH1 hardly had an effect on flowering time in the Col-0 and Ler accessions. ATH1 physically interacts with STM, BP and KNAT6 and enhances the shoot apical meristem defect of some of these genes suggesting a role in SAM maintenance. Nuclear localization is dependent upon interaction with STM.

Gene Symbol:   ATH1
Gene Name:   Homeobox gene 1
Aliases:   Not Available
RefSeq:   NM_119452
Ensembl:   AT4G32980
Entrez:   829435
Chromosome Mapping:   Not Available

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