PrimePCR™ Probe Assay: 4CL5, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0015445
Assay Design:   Exonic
Chromosome Location:   3:7451682-7451817question
Amplicon Length:   106
Splice Variants Targeted:   AT3G21230.1

Gene Information

The gene encodes a 4-coumarate coenzyme A ligase being able to use sinapate as substrate. The catalytic efficiency was in the following (descending) order: p-coumaric acid, caffeic acid, 5-OH-ferulic acid, ferulic acid and sinapic acid. At4CL5 was unable to use cinnamic acid as substrate. Knockout of At4CL5 (4cl5) revealed no effect on syringyl lignin content indicating that the activity observed does probably not occur in vivo.

Gene Symbol:   4CL5
Gene Name:   4-coumarate:CoA ligase 5
Aliases:   Not Available
RefSeq:   NM_113018
Ensembl:   AT3G21230
Entrez:   821677
UniGene:   At.38095
Chromosome Mapping:   Not Available

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