PrimePCR™ Probe Assay: SSE1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SSE1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SSE1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0013575
Assay Design:   Exonic
Chromosome Location:   2:18824260-18824374question
Amplicon Length:   85
Splice Variants Targeted:   AT2G45690.1

Gene Information

Encodes a protein with similarity to yeast Pep.6, a membrane localized protein involved in peroxisome assembly and protein-trafficking. SSE1 mutant seeds do not accumulate oils and dessicated seeds have a shrunken appearance. Involved in protein and oil body biogenesis. SSE is expressed during seed development, reaching the highest peak in mature siliques. Expression in leaves and roots is low compared to cotyledons and flowers. Located in peroxisomes and endoplasmic reticulum. Homologous to the peroxin PEX16 and complements the pex16 mutants of the yeast Yarrowia lipolytica.

Gene Symbol:   SSE1
Gene Name:   Shrunken seed protein (SSE1)
Aliases:   Not Available
RefSeq:   NM_130132
Ensembl:   AT2G45690
Entrez:   819177
Chromosome Mapping:   Not Available

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